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KMID : 0881720120270020169
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Journal of Food Hygiene and Safety
2012 Volume.27 No. 2 p.169 ~ p.175
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Evaluation of Selective Media for Isolation of Staphylococcus aureus from Agricultural Products
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Kim Se-Ri
Lee Seo-Hyun
Seo Min-Kyoung
Kim Won-Il
Park Kyoung-Hun
Yoon Hye-Jeong
Yoon Yo-Han
Yoo Soon-Young
Ryu Kyoung-Yul
Yoon Jong-Chul
Kim Byung-Seok
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Abstract
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Five kinds of selective media, such as mannitol salt agar (MSA), Baird-Parker agar (BPA), BairdParker supplemented with rabbit plasma fibrinogen (BPA+RPF), CHROMagar Staphylococcus aureus (CSA), and Petrifilm Staph Express count system (Petrifilm), were compared to recommend the optimum selective media for isolation of Staphylococcus aureus from agricultural products. Seventy four target and non target bacteria were inoculated on five selective media to analyze sensitivity and specificity. In the recovery test of injured S. aureus cells, S. aureus was exposed to acid (1% lactic acid for 10 min), heat (60o C for 90s), and cold (?20oC for 1h) conditions. And artificially contaminated agricultural products (iceberg lettuce, green pepper, and cherry tomato) was enumerated on five selective media. The sensitivity of BPA+RPF, CSA, Petrifilm, MSA, and BPA were 100%, 100%, 100%, 90.5%, 90.5%, respectively. In addition, the specificity of BPA+RPF, CSA, MSA, BPA and Petrifilm were 100%, 100%,84.6%, 75.0%, 67.3%, respectively. However, no difference among five selective media was observed in recovery on injured S. aureus cell and enumeration from agricultural products. This results suggest that BPA+RPF and CSA are the optimum media for detection of S. aureus from agricultural products.
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KEYWORD
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Agricultural products, Staphylococcus aureus, selective media, sensitivity, specificity
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